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2025 Vol.27, Issue 3 Preview Page

Research article

Study on odor-reduce effect of utilizing the Alcaligenes faecalis and Paracoccus denitrificans on solid excreta composting

가축분뇨의 퇴비화 처리 시 악취 저감을 위한 Alcaligenes faecalis 와 Paracoccus denitrificans의 활용에 관한 연구

Ju-Ho Park1
,
Do-Hyeon Lee1
,
Sin-Young Park12*
박 주호1
,
이 도현1
,
박 신영12*
1Assistant professor, Department of Animal Resources Science, Kongju National University, Chungnam 32439, Republic of Korea
2Undergraduate student, Resources Science Research Institute, Kongju National University, Chungnam 32439, Republic of Korea
1공주대학교 동물자원학과 조교수
2공주대학교 자원과학연구소 학부생
*Corresponding Author
31 December 2025. pp. 109-117
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Abstract

This study investigated the effect of microbial additives on odor reduction during the composting of pig solid manure, utilized with Alcaligenes faecalis (KACC 19159, AF) and Paracoccus denitrificans (KACC 12251, PD). Composting treatments were performed using different mixing ratios of AF and PD (T1: 7:3, T2: 5:5, T3: 3:7), while Saccharomyces cerevisiae (S), Photosynthetic bacteria (P), and Bacillus subtilis (B) were used as controls. The composting process was conducted under anaerobic conditions for 4 weeks. Odor reduction efficiency and compost quality were evaluated through pH, CIE color, moisture content, electronic nose, and sensory evaluation. The pH of the samples changed under the four typical phases of composting, indicating stable decomposition. The T3 sample exhibited a desirable color with lower redness compared to the other samples. The moisture content showed no significant differences among treatments. Volatile compounds such as dimethyl sulfide and toluene were effectively reduced in the T3 sample, as confirmed by electronic nose analysis. Sensory evaluation also demonstrated that the T3 sample received the highest scores in odor and overall appearance. In conclusion, the combined use of AF and PD, particularly at a 3:7 ratio, proved effective in reducing odors and improving compost quality during anaerobic composting of pig manure.

Keywords
Odor reduction
Environment-friendly
Pig manure
Alcaligenes faecalis
Paracoccus denitrificans
References
1

Ministry of environment., 2023. Korea. Act on the management and use of livestock excreta. Act No. 19656.

2

Kim, H.S., Park, S., Jung, W.H., Srinivasan, S., Lee, S.S., 2014. The effect of soil characters on removal of odorous gases during carcasses degradation with efficient microorganisms. Journal of the Korean Society of Environmental Engineers, 36, 277-285.

10.4491/KSEE.2014.36.4.277
3

Wang, Y.C., Han, M.F., Jia, T.P., Hu, X.R., Zhu, H.Q., Tong, Z., Lin, Y.T., Wang, C., Liu, D.Z., Peng, Y.Z., Wang, G., Meng, J., Zhai, Z.X., Zhang, Y., Deng, J.G., His, H.C., 2021. Emission characteristics of odorous substances from livestock manure. Journal of Science of the Total Environment, 145735

10.1016/j.scitotenv.2021.145735
4

Ministry of environment. Korea. Malodor prevention act. Act No. 19310.

5

Cho, K.S., Ryu, H.W., 2023. A review on livestock odor characteristics and management (I): Domestic livestock industry status, odor complaint, and management policy. Journal of Odor and Indoor Environment, 22, 90-100.

10.15250/joie.2023.22.1.90
6

Moon, Y.H., Lee, K.B., Kim, Y.J., Koo, Y.M., 2011. Current status of EM (Effective Microorganisms) utilization. Korean Society Biotechnology and Bioengineering Journal, 26, 365-373.

10.7841/ksbbj.2011.26.5.365
7

Park, C.J., Sun, S.S., 2020. Effect of dietary supplementation of enzyme and microorganism on growth performance, carcass quality, intestinal microflora and feces odor in broiler chickens. Korean Journal of Poultry Science, 47, 275-283.

10.5536/KJPS.2020.47.4.275
8

Das, S., Ho, A., Kim, P.J., 2019. Editorial: Role of microbes in climate smart agriculture. Frontiers in Microbiology, 10, 2756.

10.3389/fmicb.2019.0275631849896PMC6901993
9

Kim, K.Y., Choi, H.L., Ko, H.J., Lee, Y.G., Kim, C.N., 2005. Evaluation of odor reduction in the enclosed pig building through spraying biological additives. Journal of Animal Science and Technology, 48, 467-478.

10.5187/JAST.2006.48.3.467
10

Kang, K.H., Kam, S.K., Hu, C.G., Lee, M.G., 2006. The effect of reduction of contamins and odor according to the additives in the anaerobic maturation process of piggery slurry. Journal of Environmental Science International, 15, 169-175

10.5322/JES.2006.15.2.169
11

Jeon, S.B., Oh, H.H., Uhm, T.B., Cho, J.Y., Yang, H.J., Jeong, D.Y., 2016. Characteristic study and isolation of bacillus subtilis SRCM 101269 for application of cow manure. Korean Journal of Microbiology, 52, 74-83

10.7845/kjm.2016.6001
12

Jeonnam Bioindustry Foundation, Biocontrol Research Center. 2016. Development of photosynthetic bacteria for improvement of crop cultivation and livestock environment. Research report supported by Rural Development Administration, Korea, 1-100.

13

Lee, D.H., Jeong, S.M., Xu, X., Kim, K.B.W.R., Ahn, D.Y., 2019. Inhibition effect of bacillus subtilis on 365 nm UV-LED irradiation according to packaging materials. Microbiology and Biotechnology Letters, 47, 332-336

10.4014/mbl.1812.12006
14

Lee, S.B., Kim, J.G., Lee, D.B., Lee, K.B., Han, S.S., Kim, J.D., Baek, S.H., 2002. Changes of physico-chemical properties and microflora of pig manure due to composting with turning times and depth. Korean Journal of Soil Science and Fertilizer, 35, 127-135.

15

Yun, M.S., Song, J.Y., Park, K.H., 2005. Design of Denitrification Reactor by Using Permeabilized and Immobilized Paracoccus denitrificans. Korean Journal of Biotechnology and Bioengineering, 20,100-105.

16

Jung, T.K., Ra, C.S., Joh, K.S., Song, H.G., 2016. Characterization of heterotrophic nitrification and aerobic denitrification by Alcaligenes faecalis NS13. Korean Journal of Microbiology, 52, 166-174.

10.7845/kjm.2016.6020
17

Chen, H., Wang, Y., Lv, Y.K., 2021. Simultaneous removal characteristics of ammonium and phenol by Alcaligenes faecalis strain WY-01 with the addition of acetate. Bioprocess and Biosystems Engineering, 44, 27-38.

10.1007/s00449-020-02416-3
18

Poincelot, R.P., 1972. The biochemistry of composting. In Proceedings of the National Conference on Composting of Municipal Residues and Sludges. 1st ed. Information Transfer Inc (ed). Information Transfer Inc. Rockville. USA, 33-39.

19

Kim, H.J., Park, H.M., Lee, D.J., Kim, J.K., Lee, D.H., Jeong, K.H., 2022. Changes in characteristics of composting factors according to the presence or absence of air in pig manure compost and correlation between each factor. Journal of Animal and Environmental Sciences, 24, 99-106.

10.11109/JAES.2022.24.3.099
20

Khan, MAI., Ueno, K., Horimoto, S., Komai, F., Someya, T., Inoue, K., Tanaka, K., Ono, Yo., 2009. CIELAB color variables as indicators of compost stability. Waste Management, 29, 2969-2975.

10.1016/j.wasman.2009.06.021
21

Kwag, J.H., Choi, D.Y., Park, C.H., Jeong, K.H., Kim, J.H., Yoo, Y.H., Youn, C.K., Ra, C.S., 2008. Investigation on characteristics of swine manure of optimum volume for escalator reversing composting facility. Journal of Livestock Housing and Environment, 14, 105-112.

22

Yoo, Y.S., Shim, K.S., 2007. A study on the effective odor reduction in the area where strong civil complaints take place to odor. Gyeonggi Research Institute. Policy Research Report, No. 2007-33.

23

Ko, H.J., Kim, K., Kim, H.T., Ko, M.S., Higuchi, T., Umeda, M., 2008. Characteristic of odorous compounds emitted from livestock waste treatment facilities combined methane fermentation and composting process. Korean Journal of Animal Science and Technology, 50, 391-400.

10.5187/JAST.2008.50.3.391
24

Sirisathitkul, Y., Sirisathitkul, C., 2025. Decoding soil color: Origins, influences, and methods of analysis. Agri Engineering, 7, 58.

10.3390/agriengineering7030058
25

Jiang-ming, Z., 2017. Effect of turning frequency on co-composting pig manure and fungus residue. Journal of the Air & Waste Management Association, 67, 313-321.

10.1080/10962247.2016.1232666
Information
  • Publisher :The Korean Society of Animal Environmental Science & Technology
  • Publisher(Ko) :(사)한국축산환경학회
  • Journal Title :Journal of Animal Environmental Science
  • Journal Title(Ko) :축산시설환경학회지
  • Volume : 27
  • No :3
  • Pages :109-117
  • Received Date : 2025-09-02
  • Revised Date : 2025-11-24
  • Accepted Date : 2025-12-11
  • DOI :https://doi.org/10.11109/JAES.2025.27.3.109
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